Last data update: 24 January 2024 16:39 CET
Host name: Escherichia coli K12 WG1260 (LMBP 12432)
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| Species: | Escherichia coli |
| Group: | K12 |
| Strain designation: | WG1260 |
| Accession number: | LMBP 12432 |
| Depositor: | This host strain was deposited by Dr D.E. Culham1 and Prof. Em. Dr J.M. Wood1. 1 Department of Molecular and Cellular Biology, University of Guelph, Guelph, Canada |
| Containment level: | This strain has been assigned the containment level Class 1 following the European Directive 2009/41/EC on the contained use of genetically modified organisms, and its updates (see also the Belgian risk group classification). |
| Medium: | LB-Lennox |
| Selection marker: | - |
| Temperature: | 37°C |
| Genotype: | F- trp lacZ rpsL thi ∆putPA101 ∆(proU)600 ∆(proP-melAB)212 clsA::Tn10dTet3 proP678::Lumio |
| Phenotype: | StrR TetR |
| Properties: | - |
| Further information: | The allele cls::Tn10dTet3 was introduced into WG1259 by P1 transduction. For the construction of WG1259 the proP678::Lumio ORF was introduced into the chromosome of WG1224 by allelic exchange as described by Link et al. (1997). Resistance to streptomycin and tetracycline has been experimentally confirmed by BCCM/GeneCorner. |
| Original reference: | Romantsov et al., J. Bacteriol. 192 (2010), 912-924 [PMID: 20008071] [DOI: 10.1128/JB.00967-09] |
| Related reference: | Link et al., J. Bacteriol. 179 (1997), 6228-6237 [PMID: 9335267] [DOI: 10.1128/jb.179.20.6228-6237.1997] |
| Restricted use: | - BCCM MTA - The depositor will be informed of the customer's identity upon release of a sample outside the depositor's department. |
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Refer in your Materials and Methods: |
Escherichia coli K12 WG1260 (LMBP 12432) is available at BCCM/GeneCorner. It was deposited by Dr D.E. Culham and Prof. Em. Dr J.M. Wood and was published in Romantsov et al., 2010. |
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.